Signalling

Part:BBa_K608102:Design

Designed by: Julia Müller   Group: iGEM11_Freiburg   (2011-09-14)


CcaS, green light receptor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1685
    Illegal SpeI site found at 1780
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1685
    Illegal NheI site found at 1056
    Illegal SpeI site found at 1780
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1685
    Illegal XhoI site found at 6
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1685
    Illegal SpeI site found at 1780
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1685
    Illegal SpeI site found at 1780
    Illegal AgeI site found at 1720
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the original gene has two iGEM restriction sites, we managed to delete the SpeI cutting site, but not the EcoRI site.


Source

Amplified via PCR from genomic sequence of Synechocystis sp. PCC 6803 (Hess Lab Freiburg)

References

Our group have condon-optimized the gene sequence of this part for E.coli and deleted the EcoRI and SpeI site in the gene sequence. You could browse our webiste for detail information (https://parts.igem.org/Part:BBa_K2789001:Design)